Research Topic 1: Structure-function relationship of photosynthetic pigments
Photosynthetic pigments play important role in light energy harvesting, energy transfer, and protection against high energy irradiation (UV-Blue light) and radicals. Understanding the correlation between structure and function of pigments and its degradation products give information on the know-how of photosynthesis process at molecular level and to develop ways to optimize and enhance their potentials. In this project, we characterize pigments and pigment-protein complexes from various photosynthetic organisms. Furthermore, we are also interested in modification of the system including with nanostructures to enhance their potentials.
1. Juliadiningtyas AD, Pringgenies D, Heriyanto, Salim KP, Radjasa OK, Shioi Y, Limantara L, Brotosudarmo THP (2018) Preliminary investigation of the carotenoid composition of Erythrobacter sp. Strain KJ5 by high-performance liquid chromatography and mass spectrometry, Philippine Journal of Science 147(1): 93-100
2. Bujak, L., Olejnik, M., Brotosudarmo, T.H.P., Schmidt, M.K., Czechowski, N., Piatkowski, D., Aizpurua J., Cogdell, R.J., Heiss, W., Mackowski, S. (2014) Polarization control of metal-enhanced fluorescence in hybrid assemblies of photosynthetic complexes and gold nanorods, Physical Chemistry Chemical Physics, 16, 9015-9022
3. Luer, L., Moulisova, V., Henry, S., Polli, D., Brotosudarmo, T.H.P., Hoseinkani, S., Brida, D., Lanzani, G., Cerullo, G. and Cogdell, R.J. (2012) Tracking energy transfer between light harvesting complex 2 and 1 in photosynthetic membranes grown under high and low illumination, Proceedings of the National Academy of Sciences USA, 109(5), 1473-1478.
4. Brotosudarmo, T.H.P., Collins, A.M., Gall, A., Roszak, A.W., Gardiner, A.T., Blankenship, R.E. and Cogdell, R.J. (2011) The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium, Biochemical Journal 440, 51-61
5. Brotosudarmo, T.H.P., Kunz, R., Böhm, P., Gardiner, A.T., Moulisová, V., Cogdell R.J. and Köhler, J. (2009): Single-molecule spectroscopy reveals that individual low-light LH2 complexes from Rhodopseudomonas palustris 2.1.6. have a heterogenous polypeptide composition, Biophysical Journal, 97, 1491-1500.
6. Brotosudarmo, T.H.P., Hofmann, E., Hiller, R.G., Wörmke, S., Mackowski, S., Zumbusch, A., Bräuchle, C. and Scheer, H. (2006): Peridinin-Chlorophyll-Protein Reconstituted with Chlorophyll Mixtures: Preparation, Bulk and Single Molecule Spectroscopy, FEBS Letters, 580, 5257–5262.
Research Topic 2: Carotenoids and its degradation as bioactive compounds for functional foods:
Carotenoids are compounds known to have good antioxidant activities that can protect the body from harmful radicals as well as UV and blue light radiation. Examples of those are beta-carotene for pro-Vitamin A; lutein and zeaxanthin, known as the macular pigments, are important for eyes; and lycopene that may help protect against the development of prostate cancer. Interestingly, when carotenoid degrades it becomes into useful compounds. For example, beta-carotene degrades into beta-ionone, which is used for fragrance. Zeaxanthin degrades into crocin, which is used for pain relief compound. In this project, we are interested to develop methods for green extraction of carotenoids and its degradation products and use them as bioactive compounds for functional foods.
1. Indrawati, R., Chomiuk, A., Indriatmoko, Adhiwibawa, M.A.S., Siahaan, D., Brotosudarmo, T.H.P. and Limantara, L. (2015) Stability of palm carotenes in an organic solvent and in a food emulsion system, International Journal of Food Properties, 18(11), 2539-2548
2. Limantara L, Indrawati R, Wijaya DE, Sulistiawati E, Suparto IH, Wijayanti RDE, Brotosudarmo THP (2017) Hypocholesterolemic effect and pigments composition of herbal medicine containing higher and lower plants, International Journal of Pharmacy and Pharmaceutical Sciences, 9(11): 97-103
Research Topic 3: Developing imaging system for monitoring of biodiversity and plant health
Based on our experience working with chlorophylls and other photosynthetic pigments, we develop imaging system to monitor biodiversity and plant health. We develop home-built handheld devices, multispectral imaging spectroscopy and determination methods to capture leaves pigments and shape. We also develop method for remote sensing technology to improve sustainable management of farming. We use wide range of remote imaging technologies, including Sentinel, Landsat, RapidEyeTM, SAR, and unmanned aerial photography.
1. Prilianti, K.R., Hariyanto, S., Natali, F.D.D., Indriatmoko, Adhiwibawa, M.A.S., Limantara, L., Brotosudarmo, T.H.P (2016) Artificial neural network model for photosynthetic pigments identification using multi wavelength chromatographic data, The American Institute of Physics Conference Proceedings, 1723, 030016
2. Adhiwibawa, M.A.S., Setiawan, Y.E., Prilianti, K.R., Brotosudarmo, T.H.P. (2015) Web camera as low cost multispectral sensor for quantification of chlorophyll in soybean leaves, Proceeding of SPIE9444, doi:10.1117/12.2080951
3. Adhiwibawa, M.A.S., Setiawan, Y.E., Setiawan Y., Prilianti, K.R., Brotosudarmo, T.H.P. (2015) Application of simple multispectral image sensor and artificial intelligence for predicting of drought tolerant variety of soybean, Procedia Chemistry, 14, 246-255